Immunity against Delta and Omicron variants elicited by homologous inactivated vaccine booster in kidney transplant recipients

Background A third mRNA vaccine booster is recommended to improve immunity against SARS-CoV-2 in kidney transplant recipients (KTRs). However, the immunity against SARS-CoV-2 Ancestral strain and Delta and Omicron variants elicited by the third dose of inactivated booster vaccine in KTRs remains unknown. Methods The blood parameters related to blood cells count, hepatic function, kidney function, heart injury and immunity were explored clinically from laboratory examinations. SARS-CoV-2 specific antibody IgG titer was detected using an enzyme-linked immunosorbent assay. Cellular immunity was analyzed using interferon-γ enzyme-linked immunospot assay. Results The results showed that there were no severe adverse effects and apparent changes of clinical laboratory biomarkers in KTRs and healthy volunteers (HVs) after homologous inactivated vaccine booster. A third dose of inactivated vaccine booster significantly increased anti-Ancestral-spike-trimer-IgG and anti-Ancestral-receptor binding domain (RBD)-IgG titers in KTRs and HVs compared with the second vaccination. However, the anti-Delta-RBD-IgG and anti-Omicron-RBD-IgG titers were significantly lower than anti-Ancestral-RBD-IgG titer in KTRs and HVs after the third dose. Notably, only 25.6% (10/39) and 10.3% (4/39) of KTRs had seropositivity for anti-Delta-RBD-IgG and anti-Omicron-RBD-IgG after booster, which were significantly lower than HVs (anti-Delta-RBD-IgG: 100%, anti-Omicron-RBD-IgG: 77.8%). Ancestral strain nucleocapsid protein and spike specific T cell frequency after booster was not significantly increased in KTRs compared with the second dose, significantly lower than that in HVs. Moreover, 33.3% (12/36), 14.3% (3/21) and 14.3% (3/21) of KTRs were positive for the Ancestral strain and Delta and Omicron spike-specific T cells, which were significantly lower than HVs (Ancestral: 80.8%, Delta: 53.8%, and Omicron: 57.7%). Conclusions A third dose of inactivated booster vaccine may significantly increase humoral immunity against the Ancestral strain in KTRs, while humoral and cellular immunity against the Delta and Omicron variants were still poor in KTRs.

Clinical analysis of drug-induced liver dysfunction in COVID-19 patients in the third hospital of Wuhan in 2020

Objective: To investigate the general rules and characteristics of drug-induced liver dysfunction during the treatment of COVID-19, and to provide references for safe clinical use.

A Survey of Dummy-Based Location Privacy Protection Techniques for Location-Based Services.

As smart devices and mobile positioning technologies improve, location-based services (LBS) have grown in popularity. The LBS environment provides considerable convenience to users, but it also poses a significant threat to their privacy. A large number of research works have emerged to protect users' privacy. Dummy-based location privacy protection solutions have been widely adopted for their simplicity and enhanced privacy protection results, but there are few reviews on dummy-based location privacy protection. Or, for existing works, some focus on aspects of cryptography, anonymity, or other comprehensive reviews that do not provide enough reviews on dummy-based privacy protection. In this paper, the authors provide a review of dummy-based location privacy protection techniques for location-based services. More specifically, the connection between the level of privacy protection, the quality of service, and the system overhead is summarized. The difference and connection between various location privacy protection techniques are also described. The dummy-based attack models are presented. Then, the algorithms for dummy location selection are analyzed and evaluated. Finally, we thoroughly evaluate different dummy location selection methods and arrive at a highly useful evaluation result. This result is valuable both to users and researchers who are studying this field.

Automatic Segmentation of Novel Coronavirus Pneumonia Lesions in CT Images Utilizing Deep-Supervised Ensemble Learning Network.

Background: The novel coronavirus disease 2019 (COVID-19) has been spread widely in the world, causing a huge threat to the living environment of people. Objective: Under CT imaging, the structure features of COVID-19 lesions are complicated and varied greatly in different cases. To accurately locate COVID-19 lesions and assist doctors to make the best diagnosis and treatment plan, a deep-supervised ensemble learning network is presented for COVID-19 lesion segmentation in CT images. Methods: Since a large number of COVID-19 CT images and the corresponding lesion annotations are difficult to obtain, a transfer learning strategy is employed to make up for the shortcoming and alleviate the overfitting problem. Based on the reality that traditional single deep learning framework is difficult to extract complicated and varied COVID-19 lesion features effectively that may cause some lesions to be undetected. To overcome the problem, a deep-supervised ensemble learning network is presented to combine with local and global features for COVID-19 lesion segmentation. Results: The performance of the proposed method was validated in experiments with a publicly available dataset. Compared with manual annotations, the proposed method acquired a high intersection over union (IoU) of 0.7279 and a low Hausdorff distance (H) of 92.4604. Conclusion: A deep-supervised ensemble learning network was presented for coronavirus pneumonia lesion segmentation in CT images. The effectiveness of the proposed method was verified by visual inspection and quantitative evaluation. Experimental results indicated that the proposed method has a good performance in COVID-19 lesion segmentation.

The art reconstruction of waste ribbons based on encouraging people to overcome coronavirus disease 2019

To achieve efficient and environmental reuse of waste ribbons, artworks reconstructed from waste ribbons for decorative occasions were inspired by the dedicated and determined people working against coronavirus disease 2019 (COVID-19) and developed through a handmade and delicate strategy. With the theme of ?Ordinary * Extraordinary,? the artworks aim to convey endless positive energy and hope to people suffering from COVID-19. To fulfill the art reconstruction, the design idea, composition design and color design were comprehensively considered and carefully described. The artworks were applied for decoration in hospital wards and the home environment. Their composition and color gave medical workers, patients and ordinary people suffering from COVID-19 a sense of comfort and warmth. All positive energy from our artworks accompanied people and encouraged them never to give up and overcome COVID-19. The art reconstruction of waste ribbons proposed in this study may provide an excellent way for the reuse of waste ribbons. More importantly, it improves the added value of products and strengthens people's determination to overcome COVID-19.

Early immune responses and prognostic factors in children with COVID-19: a single-center retrospective analysis.

BACKGROUND: Early diagnostic indicators and the identification of possible progression to severe or critical COVID-19 in children are unknown. To investigate the immune characteristics of early SARS-CoV-2 infection in children and possible key prognostic factors for early identification of critical COVID-19, a retrospective study including 121 children with COVID-19 was conducted. Peripheral blood lymphocyte subset counts, T cell-derived cytokine concentrations, inflammatory factor concentrations, and routine blood counts were analyzed statistically at the initial presentation. RESULTS: The T lymphocyte subset and natural killer cell counts decreased with increasing disease severity. Group III (critical cases) had a higher Th/Tc ratio than groups I and II (common and severe cases); group I had a higher B cell count than groups II and III. IL-6, IL-10, IFN-γ, SAA, and procalcitonin levels increased with increasing disease severity. Hemoglobin concentration, and RBC and eosinophil counts decreased with increasing disease severity. Groups II and III had significantly lower lymphocyte counts than group I. T, Th, Tc, IL-6, IL-10, RBC, and hemoglobin had relatively high contribution and area under the curve values. CONCLUSIONS: Decreased T, Th, Tc, RBC, hemoglobin and increased IL-6 and IL-10 in early SARS-CoV-2 infection in children are valuable indices for early diagnosis of severe disease. The significantly reduced Th and Tc cells and significantly increased IL-6, IL-10, ferritin, procalcitonin, and SAA at this stage in children with critical COVID-19 may be closely associated with the systemic cytokine storm caused by immune dysregulation.

PANDA: Policy-aware Location Privacy for Epidemic Surveillance (preprint)

In this demonstration, we present a privacy-preserving epidemic surveillance system. Recently, many countries that suffer from coronavirus crises attempt to access citizen's location data to eliminate the outbreak. However, it raises privacy concerns and may open the doors to more invasive forms of surveillance in the name of public health. It also brings a challenge for privacy protection techniques: how can we leverage people's mobile data to help combat the pandemic without scarifying our location privacy. We demonstrate that we can have the best of the two worlds by implementing policy-based location privacy for epidemic surveillance. Specifically, we formalize the privacy policy using graphs in light of differential privacy, called policy graph. Our system has three primary functions for epidemic surveillance: location monitoring, epidemic analysis, and contact tracing. We provide an interactive tool allowing the attendees to explore and examine the usability of our system: (1) the utility of location monitor and disease transmission model estimation, (2) the procedure of contact tracing in our systems, and (3) the privacy-utility trade-offs w.r.t. different policy graphs. The attendees can find that it is possible to have the full functionality of epidemic surveillance while preserving location privacy.